蛋白质组学详细

蛋白质/蛋白修饰鉴定 > 蛋白质定性研究

    蛋白质定性研究

            质谱是进行蛋白质定性研究最主要的技术手段，在二维电泳胶点、IP/co-IP/pull-down产物和shotgun混合蛋白质的蛋白序列和修饰位点定性鉴定工作中发挥核心作用。

     

            华盈生物采用纳升液相色谱-四级杆飞行时间串联质谱（分辨率：40000 FWHM、扫描速度：50张全谱/秒）进行蛋白质定性检测，其鉴定精确度远超传统的MALDI-TOF/TOF等技术，能够大大提高鉴定蛋白质的数量和准确性。


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    技术路线

            


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    主要仪器设备


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    样品要求

     

                           

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    应用方向


                         ◇  蛋白质鉴定

                         ◇  蛋白质全谱分析

                 

    客户代表文章


    1. Chen XJ, et al. Homoharringtonine deregulates transcriptional expression by directly binding NF-κB repressing factor. Proc Natl Acad Sci U S A. 2019 Feb 5;116(6):2220-2225.（IF=9.504，上海交大医学院附属瑞金医院）

    2. Li R, et al. Expressway to Rheumatoid Arthritis by Macrophage-derived Microvesicle-Coated Nanoparticles. Nano Lett., 2018. (IF=12.08，复旦大学)

    3. Huang H, et al. Kisspeptin/GPR54 signaling restricts antiviral innate immune response through regulating calcineurin phosphatase activity. Science Advances, 2018, 4(8): eaas9784. (IF=11.5，华东师范大学)

    4. Yang L,  et al. Lectin Microarray Combined with Mass Spectrometry Identifies Haptoglobin-Related Protein (HPR) as a Potential Serologic Biomarker for Separating Nonbacterial Pneumonia from Bacterial Pneumonia in Childhood. Proteomics Clin Appl, 2018, 12: e1800030.（IF=3.567，上海儿童医学中心）

    5. Jiang P, et al. Quantitative proteomics analysis of differentially expressed proteins in ruptured and unruptured cerebral aneurysms by iTRAQ. Journal of Proteomics, 2018, 182. (IF=3.722，首都医科大学附属北京天坛医院)

    6. Sun H M, et al. PALLD Regulates Phagocytosis by Enabling Timely Actin Polymerization and Depolymerization. J Immunol. 2017 Sep 1; 199(5): 1817-1826.  (IF=4.539，上海交大医学院附属瑞金医院)

    7. You D, et al. Acetyl coenzyme A synthetase is acetylated on multiple lysine residues by a protein acetyltransferase with a single Gcn5-type N-acetyltransferase (GNAT) domain in Saccharopolyspora erythraea. J Bacteriol. 2014 Sep; 196(17): 3169-78. (IF=3.219，华东理工大学)

     

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